2017-05-10 12:03:022025-05-07 09:24:11
title
kipA
pxpC
description
antagonist of KipI, control of the phosphorelay
subunit of ATP-dependent 5-oxoprolinase, antagonist of [[protein|PxpB]], control of the [SW|phosphorelay]
locus
BSU04090
BSU_04090
proteinLength
337
335
geneLength
1011
1008
function
control of the phosphorelay, initiation of sporulation
detoxification of 5-oxoproline, control of the [SW|phosphorelay], initiation of [SW|sporulation]
product
antagonist of KipI
subunit of 5-oxoprolinase, antagonist of [SW|KipI]
names
kipA
pxpC
kipA
outlinks
bsu
BSU04090
BSU_04090
Gene
Coordinates
460,592 → 461,599
460,592 461,599
The protein
Structure
[PDB|5DUD] (from E. coli, 36% identity)
[PDB|5DUD] ( the YbgJ-YbgK complex from E. coli, 36% identity)
References
Gene
Phenotypes of a mutant
no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]
reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]
The protein
Catalyzed reaction/ biological activity
5-oxoproline + ATP --> glutamate + ADP + Pi [pubmed|28830929]
The protein
Protein family
PxpC family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Kinetic information
Km (5-oxoproline): 39 M [pubmed|28830929]
Biological materials
Mutant
BKE04090 ([[gene|pxpC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG
BKK04090 ([[gene|pxpC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG